Veterinary Laboratory Procedures II

Curriculum Guideline

Effective Date:
Course Code
AHTT 1202
Veterinary Laboratory Procedures II
Animal Health Technology
Science & Technology
Start Date
End Term
Not Specified
Semester Length
15 weeks
Max Class Size
Contact Hours
6 hours/week: 3 hour lecture/3 hour lab
Method Of Instruction
Methods Of Instruction

This course involves two hours per week of classroom instruction and three hours per week of laboratory activity.

Course Description
This course will further develop veterinary clinical and laboratory skills, including serum chemistry testing, urinalysis, and tissue and fluid collection and analysis. Cytological techniques and microbiology are reviewed
for all relevant species, with the emphasis being on pathogens relevant to companion (small) animals. Included
will be a review of mathematics necessary for success in the laboratory and in test interpretation. The detailed review of veterinary laboratory procedures is completed in this course.

Enrollment is limited to students of the Animal Health Technology Program
Course Content

The major topics in the course include the following:

1. Clinical Pathology Part II:

- canine and feline blood types

- blood typing techniques

- cross matching of blood for transfusion


2. Clinical Pathology Part III:

a.  Immunological and serological testing

-  ELISA, ICT, serum agglutination and precipitation tests

-  understanding serum protein electrophoresis

-  theoretical and practical applications of antibody titers

-  in clinic test kits (snap, micro well) for heartworm and FeLV/FIV


b.  Whole blood and Serum Chemistry

-  sample collection and handling

-  record keeping and lab requisition forms

-  understanding testing procedures

-  quality control

-  manual testing


c.  Function and specialty testing

-  thyroid gland function

-  adrenocortical gland function

-  glucose homeostasis

-  hepatic function testing

-  renal function testing


3. Clinical Pathology Part IV:


Components of the Urinalysis

-  sample collection techniques

-  gross evaluation

-  determining urine specific gravity and significance

-  biochemical analysis

-  sediment preparation and analysis


4. Clinical Pathology Part V:


a.  Cytology

-  tissue sampling and collection techniques

-  preparation and staining

-  gross and microscopic tissue and fluid analysis


b.  Histopathology

-  tissue sample collection and preservation


5. Microbiology:

-  pathogen (bacterial, viral, fungal) growth patterns, morphology, testing techniques and control methods

-  sample collection, including use of culture media

-  gross colony and microscopic organism evaluation, with coverage of staining techniques

-  biochemical analysis

-  laboratory safety and quality control

-  basics of antibiotic therapy and applications to common veterinary pathogens

Learning Outcomes

Upon completion of Veterinary Laboratory Procedures II AHTT 1202, the student will be able to:

1.  Demonstrate accuracy in performing basic laboratory mathematical calculations.

2.  Describe the common canine and feline blood types, and understand the technique for blood typing.

3.  Understand the application and methodology of determining serum chemistry profiles and function testing routinely used in veterinary medicine. 

4.  Demonstrate knowledge of common serological and immunological veterinary testing procedures.

5.  Perform a complete urinalysis.

6.  Display competency with the collection, handling and preparation of tissue and fluid samples for analysis.

7.  Perform a microscopic examination of cytological and spermatozoa samples, with the ability to identify common cell types.

8.  Realize the significance of more common microbiological veterinary pathogens.                                                                                                                                  

9.  Apply tissue staining techniques and usage of microbiological culture media to identify microscopic organisms.

Means of Assessment

Means of Assessment:


Written Assignments                                                                              10-20%

Laboratory Assignments & Projects                                                           15-20

Quizzes                                                                                                10-20             

Midterm                                                                                                25

Professionalism (Preparation, Participation & Attendance)                           10

Final Examination                                                                                  25


Grades:      A+ 95-100,      A 90-94,      A- 85-89,      B+ 80-84,      B 75-79,      B- 70-74,


                   C+ 65-69,         C 60-64,      C- 55-59,      P 50-54,        F 0-49.



Textbook Materials

Textbooks and Materials to be Purchased by Students:

  1. Foreyt, W.J., 2001, Veterinary Parasitology Reference Manual. Blackwell Publishing, 5th ed. (required)
  2.  Hendrix, C.M., 2002, Laboratory Procedures for Veterinary Technicians. Mosby, 4th ed. (required)

McCurnin, D.M. & Bassert, J.M., 2010, Clinical Textbook for Veterinary Technicians. Elsevier, 7th ed. (required)

Which Prerequisite